Fig 1: PKIa increased the expression of TJ proteins and reduced TSLP and IL-33 production in HaCaT cells.a–f PKIa, CLDN-1, occludin, ZO-1, CLDND1, and CLDN16 expression was analyzed with western blotting after cells were treated with PKIa siRNA. The expression was quantified relative to GAPDH expression with a ChemiScope analysis (means ± SD; n = 3; *P < 0.05, **P < 0.01, ***P < 0.001 versus negative siRNA). g Immunofluorescent quantification of CLDN-1 expression (n = 3; magnification: ×200; scale bar: 100 µm). h Immunofluorescent quantification of CLDN-1, occludin, and ZO-1 expression (n = 3; magnification: ×200; scale bar: 100 µm). i–m CLDN-1, occludin, CLDND1, and CLDN16 expression was analyzed with western blotting and quantified relative to GAPDH expression with a ChemiScope analysis (n = 3). n, o TSLP and IL-33 production was measured with ELISAs (means ± SD; n = 6; ###P < 0.001 versus control, **P < 0.01, ***P < 0.001 versus TNF-a). p–r CLDN-1 and occludin expression was analyzed with western blotting and quantified relative to GAPDH expression with a ChemiScope analysis (means ± SD; n = 3; #P < 0.05, ###P < 0.001 versus control, *P < 0.05, ***P < 0.001 versus miR-155-5p mimic).